Isoelectric Point Separations of Peptides and Proteins

Isoelectric Point Separations of Peptides and Proteins

The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also dis...

Better Separations for Proteins and Peptides by

Natural peptides and proteins: potent tyrosinase inhibitors

Background and objectives: Tyrosinase is a copper containing oxidase which is crucial for controlling the production of melanin in creatures such as bacteria, fungi, plants and mammals. It is involved in the first two steps of melanin biosynthesis and leads to pigmentation and different types of cancer such as melanoma. Also, it is responsible for browning of fruits and vegetab...

Optimizing Conditions for Capillary Isoelectric Focusing Separations Using

The Isoelectric Point of Adsorbed Hemo- Globin*

It is well established that various proteins, as gelatin and albumin, can be adsorbed on various adsorbents so that the proteincoated particle of adsorbent behaves electrophoretically as a particle of protein (Loeb, 1923; Freundlich and Abramson, 1928). Dummett and Bowden (1933) have recently reported, however, that the behavior of adsorbed hemoglobin varies with the adsorbent surface. Thus, wh...